Automating Cell Line Development With C-STATION Powered By GBG

Automating Cell Line Development
with C.STATION Powered by GBG

Thursday, November 30

11:00 AM - 12:00 PM EST

Virtual

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Patrick Weindl holds a degree in Biotechnology and has a keen interest in the integration of cutting-edge technologies within laboratory settings. With extensive experience in benchtop device development, he has honed his skills in hardware design, embedded electronics, and semi-automated software, particularly in the sectors of aerosol toxicology and pharmaceuticals. In early 2022, Patrick joined CYTENA as the Product Owner of the C.STATION. In this role, he is responsible for overseeing the technical implementation of hardware, ranging from robotics to sterility measures, as well as leading the development of a comprehensive software suite. This suite enables CLD scientists to concentrate on core research activities, alleviating the burden of routine tasks.

Dustin Whitson is an Applications Consulting Scientist at Biosero where he works with scientists from labs around the world to design solutions for automating science. With 8 years of experience as a bench scientist in both research and forensic labs, Dustin understands firsthand what labs need from their automation solutions. He has spent the last 6 years specializing in delivering automation into the lab setting with applications experience at Biosero and Hamilton Robotics.

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FAQ

Should I choose the C.STATION for developing an industrial cell line aimed at high-yield antibody production?

Yes. The C.STATION configuration “Suspension BSL1” has been specifically designed to run Cell Line Development campaigns to select high producer clones of antibody and antibody-like molecules. In addition, the Suspension BSL1 (FB) also enables shaken cultures and an embedded Fed-batch assay, which are usually employed with suspension producer cell lines.

What components are included in the C.STATION system?

The C.STATION consists of CYTENA UP.SIGHT (Dispensing + Imaging), Hamilton STARlet (Liquid Handling), Brooks Automation PF400 (X) (Plate Handling), ThermoFisher Scientific Cytomat 2 (Incubation), QInstruments BioShake 3000 elm (Plate Shaking).

What types of plates are compatible with the system, including both shallow and deep well formats (such as 96, 384, etc.)?

All C.STATIONs: up to 28 mm plate height, e.g. 384/96/48/24/12/6-Well plates
With fed-batch option: up to 47 mm plate height, e.g. 96/48/24/12/6-Deepwell plates

How does the device handle different liquid classes, including varying viscosities and the presence of debris?

Liquid classes are set automatically based on the project schedule, the task to be carried out (for example, they can differ between media replenishments and clone transfers), and various parameters (such as tip size, transfer volume etc.) as defined in the liquid handling editor UI.

Is it possible to select high-producer clones using fluorescence readouts with the C.STATION?

Yes. The UP.SIGHT on the C.STATION can be equipped with a fluorescence bottom-plate imager module. Antibody and antibody-like titers can be measured with the UP.SIGHT in combination with the F.QUANT assay.

What types of molecules has the C.STATION been qualified with, including monoclonal antibodies, bi-specifics, tri-specifics, etc.?

The C.STATION is molecule-agnostic to a large extent. The workflows can be freely configured to fit the specific needs of each development campaign. Our customers use the C.STATION for development of different types of molecules. CYTENA typically validates and develops features employing mAB-producing cell lines.

How does the C.STATION manage lids for deep well plates, and does it support stainless steel lids?

In general, all lids are handled similarly. The C.STATION, in principle, supports all labware with a maximum height of 47 mm that conforms to ANSI-SLAS 1/2-2004 (R2012) standards and has a weight of less than 800 g for regular handling. In fed-batch workflows, the combined weight of 7 plates should not exceed 850 g during shaken incubation at 1200 rpm.

Which device is utilized for cell counts within the C.STATION?

Cell count is performed on the C.STATION by bottom-plate imaging with the UP.SIGHT and cell count analysis of the obtained images with C.STUDIO.

What manual interventions are required when operating the C.STATION?

Manual interventions are limited to planning and initiating campaigns, as well as supplying consumables such as cartridges for single-cell dispensing, racks with pipette tips, reagents, and empty well plates.

Is in-house expertise required to maintain the GBG software, and what provisions are there for software modifications?

No, the C.STATION program does not require in-house expertise, as it is tailored to allow operators to focus on their application, workflow, and results. By design, the software on the C.STATION offers numerous options for parametrization, including aspects such as schedules, workflows, liquid handling, imaging, and dispensing.

Can you provide information on the estimated maintenance costs for C.STATION?

With its unique architecture, the C.STATION requires only one service contract, which covers all CYTENA support services. This includes, but is not limited to, a specified allocation of yearly remote support, on-site visits, and yearly preventive maintenance. Please contact us for more details.

Can you provide information on the estimated cost for consumables, such as plates, for the device?

The cost of consumables largely depends on the specific types of plates used. Additionally, operators can design workflows, which will influence the number of required plates and other consumables. Contact CYTENA for labware recommendations based on our validation studies, or for an estimate of consumables used per project, depending on the workflow

Does the C.STATION support automated pipettors other than those from Hamilton?

No, however, Green Button Go simplifies the process of automating a range of liquid handlers within a customized work cell. For more information, please contact Biosero.

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Revolutionize your cell line development workflow across multiple research areas. Focus on your research as our dispenser ensures precision and efficiency, making it the perfect tool for both complex experiments and simple assays. Experience the future of cell line development today.