Microplate agitation culture systems use positive and negative air pressure to control fluid flow into and out of each well at different speeds and patterns. This enables reciprocal, pipetting-like mixing, ensures a constant supply of oxygen, and maintains a homogeneous, healthy cell culture environment. Various sensors automatically adjust and regulate conditions, such as temperature and CO₂ levels, to provide an optimal incubation environment for successful cell culture.

These systems reduce time and costs associated with raw materials and labor by:

• Shortening the clone expansion and recovery phase, allowing faster identification of the best clones.
• Optimizing culture conditions more efficiently, accelerating process development.

The working volume of microplate agitation culture systems ranges from 150 to 1600 microliters, enabling small-scale agitation cultures and the screening of clones or expensive reagents before progressing to lab-scale productivity.

Microplate agitation culture systems can cultivate a variety of cell types, from CHO cell lines commonly used in cell line development to patient-derived blood cells and complex spheroids/organoids.

Microplate agitation culture systems are a type of microfluidic system that offers exceptional control over fluid flow, enabling precise manipulation at the microscale. This precision is particularly advantageous for small-scale experimentation and delicate cell cultures.